Biology of Human Tumors LIM and SH3 Protein 1 Induces TGFb-Mediated Epithelial–Mesenchymal Transition in Human Colorectal Cancer by Regulating S100A4 Expression
نویسندگان
چکیده
Purpose: The expression of LIM and SH3 protein 1 (LASP1) was upregulated in colorectal cancer cases, thereby contributing to the aggressive phenotypes of colorectal cancer cells. However, we still cannot decipher the underlying molecular mechanism associated with colorectal cancer metastasis. Experimental Design: In this study, IHC was performed to investigate the expression of proteins in human colorectal cancer tissues.Western blot analysiswas used to assess the LASP1-induced signal pathway. Two-dimensional difference gel electrophoresis was performed to screen LASP1-modulated proteins and uncover the molecular mechanism of LASP1. TGFb was used to induce an epithelial–mesenchymal
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